Expression and Characterization ofCoprothermobacter proteolyticusAlkaline Serine Protease
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چکیده
منابع مشابه
Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8-10). In addition, the enzyme had an elevated optimum temperature...
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ژورنال
عنوان ژورنال: The Scientific World Journal
سال: 2013
ISSN: 1537-744X
DOI: 10.1155/2013/396156